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KMID : 0382319950150020088
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Environmental Mutagens and Carcinogens
1995 Volume.15 No. 2 p.88 ~ p.93
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Sensitization Effects of Hyperthermia on Bleomycin-induced DNA Strand Breaks and Replication Inhibition in CHO-K1 Cells in Vitro
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김찬길/Kim CG
김현숙/박상대/Kim HS/Park SD
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Abstract
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Effects of hyperthermia on the induction of DNA single strand breaks and replication inhibition were studied in bleomycin-treated CHO-K cells by alkaline elution and alkaline sucrose gradient sedimentation. Bleomycin-induced DNA single strand breaks of DNA were dose-and time-dependently increased, and these strand breaks of DNA were gradually rejoined as post-incubation time passed. Treatment with hyperthermia alone did not affect the induction of DNA single strand breaks. However, pre-exposure of cells to hyperthermia followed by bleomycin treatment greatly increased the single strand breaks, and also reduced the rejoining processes of bleomycin-induced DNA single strand breaks. Bleomycin selectively inhibited the replicon initiation. The combined treatment with hyperthermia and bleomycin markedly potentiated the nonspecific inhibition of replication.
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